肾病综合征合并肺栓塞的临床研究进展

肾病综合征是一种临床常见疾病,其患者体内常呈高凝状态,极易发生血栓栓塞事件,而其中以肾静脉血栓、肺动脉栓塞和 下肢深静脉血栓最为常见。由于肺动脉栓塞早期缺乏特征性的临床表现,病情隐匿,所以极易误诊或漏诊,发现时患者病情往往 已十分严重,致死率极高。目前,对于肾病综合征合并肺栓塞的发生率国内外报道不一,尚无准确的流行病学资料,而对于其发病 原因、危险因素、早期诊断及是否需要预防性抗凝治疗等均存在争议,本文主要结合文献对肾病综合征合并肺栓塞的流行病学、 病因及发病机制、诊断、高危因素和治疗进行了综述,尤其是对目前争议较大的肾病综合征合并肺栓塞患者是否需要早期抗凝治 疗。     

A novel Werner Syndrome mutation: pharmacological treatment by read-through of nonsense mutations and epigenetic therapies

Werner Syndrome (WS) is a rare inherited disease characterized by premature aging and increased propensity for cancer. Mutations in the WRN gene can be of several types, including nonsense mutations, leading to a truncated protein form. WRN is a RecQ family member with both helicase and exonuclease activities, and it participates in several cell metabolic pathways, including DNA replication, DNA repair, and telomere maintenance. Here, we reported a novel homozygous WS mutation (c.3767 C > G) in 2 Argentinian brothers, which resulted in a stop codon and a truncated protein (p.S1256X). We also observed increased WRN promoter methylation in the cells of patients and decreased messenger WRN RNA (WRN mRNA) expression. Finally, we showed that the read-through of nonsense mutation pharmacologic treatment with both aminoglycosides (AGs) and ataluren (PTC-124) in these cells restores full-length protein expression and WRN functionality.     

猪瘟和猪繁殖与呼吸综合征混合感染的快速检测及地域流行性分析

呼吸道和繁殖障碍疾病是猪场最常见的两种疾病,严重影响猪群的健康和正常生产,已经给世界养猪业造成了很大的经济损失。而且这两种疾病的病原复杂多样,不同的猪场病因可能完全不同,从而给疾病的诊断与防治带来了极大的困难。多年的研究表明,呼吸道和繁殖障碍疾病的病原包     

32株SARS冠状病毒基因组比对在PCR检测中的应用

进行了32株SARS冠状病毒基因组的多序列比对分析.所得无根进化树揭示SARS冠状病毒之间的同源性.同时,验证了所设计的PCR检测引物对全部32株SARS冠状病毒检测的适用性.     

对虾白斑综合症病毒重组cDNA克隆的构建与分析

取经人工注射感染了对虾白班综合症病毒40-45h的凡纳对虾鳃组织,分离mRNA,以mRNA为模板合成双链cDNA,并克隆于PUC质粒的Not I/Sal I位点,构建了1000余株对虾感染后期鳃细胞的重组cDNA克隆,重组质粒经PCR鉴定插入片段,DNA斑点杂交分析目的片段,测定了20株对虾白斑综合症病毒的重组cDNA克隆的末端DNA序列,并对其进行了包含存在的开放阅读框架,启动区上游序列、编码产物的特性等分析。结果显示,PCR产物在0.3-1.6kb之间;大于1kb的克隆中有31.8%的克隆为白斑综合症病毒的重组cDNA克隆。已测序的不包含同源序列的13株克隆中含有14个开放阅读框,其中11个上游可检出启动子基序,4个可检出启动子调制元件,ORF转译产物的特性基序分析显示,有2个ORFs可检出锌指基序,3个ORFs可检出亮氮酸拉链基序,2个ORFs可检出NTP结合基序,未检定核定位信号基序。     

NHS-A isoform of the NHS gene is a novel interactor of ZO-1

Mutations in the NHS (Nance-Horan Syndrome) gene lead to severe congenital cataracts, dental defects and sometimes mental retardation. NHS encodes two protein isoforms, NHS-A and -1A that display cell-type dependent differential expression and localization. Here we demonstrate that of these two isoforms, the NHS-A isoform associates with the cell membrane in the presence of intercellular contacts and it immunoprecipitates with the tight junction protein ZO-1 in MDCK (Madin Darby Canine Kidney) epithelial cells and in neonatal rat lens. The NHS-1A isoform however is a cytoplasmic protein. Both Nhs isoforms are expressed during mouse development. Immunolabelling of developing mouse with the anti-NHS antibody that detects both isoforms revealed the protein in the developing head including the eye and brain. It was primarily expressed in epithelium including neural epithelium and certain vascular endothelium but only weakly expressed in mesenchymal cells. In the epithelium and vascular endothelium the protein associated with the cell membrane and co-localized with ZO-1, which indirectly indicates expression of the Nhs-A isoform in these structures. Membrane localization of the protein in the lens vesicle similarly supports Nhs-A expression. In conclusion, the NHS-A isoform of NHS is a novel interactor of ZO-1 and may have a role at tight junctions. This isoform is important in mammalian development especially of the organs in the head.     

Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster

The fetus has a high requirement for cholesterol and synthesizes cholesterol at elevated rates. Recent studies suggest that fetal cholesterol also can be obtained from exogenous sources. The purpose of the current study was to examine the transport of maternal cholesterol to the fetus and determine the mechanism responsible for any cholesterol-driven changes in transport. Studies were completed in pregnant hamsters with normal and elevated plasma cholesterol concentrations. Cholesterol feeding resulted in a 3.1-fold increase in the amount of LDL-cholesterol taken up by the fetus and a 2.4-fold increase in the amount of HDL-cholesterol taken up. LDL-cholesterol was transported to the fetus primarily by the placenta, and HDL-cholesterol was transported by the yolk sac and placenta. Several proteins associated with sterol transport and efflux, including those induced by activated liver X receptor, were expressed in hamster and human placentas: NPC1, NPC1L1, ABCA2, SCP-x, and ABCG1, but not ABCG8. NPC1L1 was the only protein increased in hypercholesterolemic placentas. Thus, increasing maternal lipoprotein-cholesterol concentrations can enhance transport of maternal cholesterol to the fetus, leading to 1) increased movement of cholesterol down a concentration gradient in the placenta, 2) increased lipoprotein secretion from the yolk sac (shown previously), and possibly 3) increased placental NPC1L1 expression.     

失眠症的中医证候分析与中药治疗概况

失眠症是指持续相当长时间的对睡眠的质和量不满意的状况,可引起精神无法集中、思考能力减退、警觉力与判断力下降、免疫功能低下、内分泌紊乱等,还与感冒、抑郁症、糖尿病、肥胖、中风、心脏病和癌症的发生有关。现代社会,随着生活水平的提高,体力劳动减少,脑力劳动增加,工作及精神压力日益加重,很多失眠患者情志不畅的表现尤为突出。经过临床观察,我们发现五脏气机失调在失眠症的辨证论治中占有主导作用,因此对失眠及失眠症的研究与治疗就成为重要课题。     

属性层次模型在中医证候诊断中的应用

目的：研究一种综合考虑专家主观经验和机器学习客观规则的中医证候诊断模型。方法：基于属性层次分析模型,提出了一种可以应用于中医证候识别的方法,并编程实现了智能系统。系统融合中、西医多个特征构建决策属性,集成中医专家主观经验和机器学习客观规则联合辨证,实现了离散属性测度、相对属性权和合成属性权的计算方法。结果：构建了中医证候诊断系统,针对肝硬化样本病例学习辨证规则,并识别新样本的证候。结论：通过中医肝硬化数据进行系统的实例验证研究,表明该系统所得辨证结果与临床诊断吻合较好。     

IL-6、IL-23在肠易激综合征患者肠黏膜的表达及意义

目的：研究肠易激综合征（irritable bowel syndrome,IBS）患者回肠末端及升结肠黏膜组织中白介素-6（interleukin-6,IL-6）、白介素-23（interleukin-23,IL-23）的表达。方法：70例腹泻型和40例便秘型IBS患者及30例健康人,用免疫组化法测定回肠末端及升结肠肠黏膜中IL-6及IL-23水平。结果：IL-6及IL-23在腹泻型及便秘型IBS患者回肠末端及升结肠的表达均高于对照组（P〈0.05）。结论：IBS患者回肠及升结肠黏膜炎性细胞因子表达水平升高,可能与IBS症状有关。